Integrative structural biology: The exocyst interactions in 3D
Understanding cellular functions requires capturing the spatiotemporal organization of dynamic protein interaction networks. We aim to map the spatiotemporal arrangement of protein assemblies that regulate vesicle tethering during constitutive exocytosis. By integrating in situ observations from PICT experiments with structural data, we generate dynamic 3D models that help us to elucidate the molecular mechanisms underlying vesicle tethering.
Structural cell biology: Cell mechanisms in situ
The study of the mechanisms that mediate the cell’s biology has been traditionally impaired by the complexity and dynamism of the machinery involved. Indeed, the characterization of short-lived complexes remains as one of the major bottlenecks for modern structural biology. We combine the measurement of protein spatial organization from SMLM, membrane ultrastructure from cryo-ET and dynamics from particle tracking to resolve the higher-order mechanisms that control membrane dynamics.
Evolutionary cell biophysics: modelling principles in cell adaptation
What are the universal principles that control cell adaptation to environmental changes? Evolutionary patterns have traditionally been studied at a population level, from Darwin’s phenotypic characterizations to large-scale genomics. However, little is known about the biophysical principles that dictate how an organism’s functionality is preserved whilst adapting to specialized environments. We combine live-cell imaging, genetics, and biophysical modelling to uncover molecular mechanisms that preserve fundamental processes under environmental stresses. Adaptation to pH, temperature, and osmolarity are investigated in both model and non-model yeast species, the latter being isolated from high-altitude Pyrenean ecosystems. This molecular perspective allows us to generate quantitative understanding of adaptation and resilience in the face of environmental fluctuations.
